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acer truncatum seed oil

doi: 10.1111/acel.12127, Jolitha, A. Moreover, long-term dietary -linolenic acid supplement improved learning and memory impairments of naturally aging rats (Gao et al., 2016). (2003). BDNF binding tropomyosin receptor kinase B (TrkB) activated auto-phosphorylation on the tyrosine residues of TrkB in the intracellular region, resulting in the activation of multiple signaling cascades including the PI3K-AKT pathway, the phospholipase C1 (PLC1)-PKC pathway and the MAPK (mitogen-activated protein kinases) -ERK (extracellular signal-regulated kinase) pathway (Longo and Massa, 2013; Guo et al., 2019). 188, 140154. Acer truncatum seed oil administration activated BDNF signal pathway in aging mice. Dysregulation of Myosin Complex and Striated Muscle Contraction Pathway in the Brains of ALS-SOD1 Model Mice.

From discoveries in ageing research to therapeutics for healthy ageing. 23, 201205. Drug. This work was carried out at Centro de Pesquisa Agropecuria de Clima TemperadoCPACT/EMBRAPA, PelotasRS, Brazil, during 2018 Interaction of DGAT1 and PDAT1 to enhance tag assembly in Arabidopsis Plant Signal. Hort. Alotaibi, S.S, Elseehy, M.M., Aljuaid, B.S. 37 1066 1084 doi: 10.1007/s00344-017-9769-4, Zhang, Q.Y., Yu, R., Sun, D.Y., Raham, M., Xie, L.H., Hu, J.Y., He, L.X., Kilaru, A., Niu, L.X. Antioxid Redox. De novo transcriptome analysis of an imminent biofuel crop, L. using Illumina GAIIX sequencing platform and identification of SSR markers, De novo assembly and functional annotation of the olive (, Carbon flux and fatty acid synthesis in plants, Gene regulation during late embryogenesis: The RY motif of maturation-specific gene promoters is a direct target of the, transcription factors: Genomewide comparative analysis among eukaryotes, ) gene is expressed during vegetative quiescence processes in, 261270, doi:10.1046/j.1365-3040.1999.00428.x, MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods, Time-series analyses of transcriptomes and proteomes reveal molecular networks underlying oil accumulation in canola, DEGseq: An R package for identifying differentially expressed genes from RNA-seq data, Metabolic interactions between the lands cycle and the Kennedy pathway of glycerolipid synthesis in, Comparative transcriptome analysis two genotypes of, Bunge seeds reveals candidate genes that influences seed VLCFAs accumulation, Integrated analysis of transcriptomic and proteomic data from tree peony (, ) seeds reveals key developmental stages and candidate genes related to oil biosynthesis and fatty acid metabolism, A new resource of nervonic acid from purpleblow maple (, Transcriptome analysis of metabolic pathways associated with oil accumulation in developing seed kernels of, Response surface methodology optimization of ultrasonic-assisted extraction of, leaves for maximal phenolic yield and antioxidant activity, Fruit spray of 24-epibrassinolide and fruit shade alter pericarp photosynthesis activity and seed lipid accumulation in, Comparative transcriptome analysis reveals an efficient mechanism for -linolenic acid synthesis in tree peony seeds, FUSCA3 activates triacylglycerol accumulation in, Bunge: A natural inhibitor of fatty acid synthase with antitumor activity, iTAK: A program for genome-wide prediction and classification of plant transcription factors, transcriptional regulators, and protein kinases, Journal of American Society for Horticultural Science, Diversity & Inclusion Statement/Code of Ethics, Journal of the American Society for Horticultural Science. Evidence suggests that -9 FAs such as NA participate in processes of cognitive improvement; however, their mechanism remains ambiguous. In general, the regulatory mechanism of FA metabolism is complicated and may need several TFs to work together. The heat map is displayed according to log10 [fragments per kilobase per million (FPKM) + 1] values. Indeed, inflammation had recently been proposed as one of the important symptoms of aging (Zhang et al., 2017). In order to explore the molecular mechanism of the seed oil improving learning and memory of aging mice, proteomics technology was used to analyze the proteins expression in the hippocampus. 146, 2; 10.21273/JASHS05013-20. MCODE analysis indicated the DE proteins in the oil treated aging mice were related to complement and coagulation cascades, inflammatory response and innate immune response. Psychiatry. Notably, in the 180 vs. 85 DAF sample (when desiccation occurs and the levels of FAs start to decrease), the response to stress and degradation complex (peptidase, hydrolase activity) terms were enriched. 124, 388396. Primers (53) used in the quantitative real-time polymerase chain reaction (qRT-PCR) to validate the reliability of the RNA-seq results. doi: 10.1016/S0197-4580(03)00112-X, Erickson, K. I., Miller, D. L., and Roecklein, K. A. One-way ANOVA followed by Tukeys multiple comparison tests. 8, 1830. Antioxidants | Free Full-Text | Effective Improvement of the Oxidative Stability of Acer truncatum Bunge Seed Oil, a New Woody Oil Food Resource, By Rosemary Extract | Notes Journals Antioxidants Volume 12 Issue 4 10.3390/antiox12040889 Version Notes Open Access Article Article Versions Notes Webseed oil of 138 accessions of 14 . doi: 10.1007/s10522-008-9154-6, Joubert, C., and Chainay, H. (2018). doi: 10.2147/CIA.S165399, Klencklen, G., Despres, O., and Dufour, A. Polyunsaturated acids such as DHA and omega-3, have been widely reported to improve learning and memory on animals, but marine organisms are the major source for DHA and Omega-3 (Cholewski et al., 2018). 9:680386. doi: 10.3389/fcell.2021.680386. 2016 iTAK: A program for genome-wide prediction and classification of plant transcription factors, transcriptional regulators, and protein kinases Mol. Detected PPI modules for aging related proteins. Mol. Exp. Based on frost dates and planting zones. doi: 10.1016/j.exger.2006.04.007, Jolitha, A. The clean reads were mapped to the A. truncatum genome we sequenced previously (Ma et al., 2020), with an average mapping percentage of 90.39% per sample. doi: 10.1177/1073858410397054, Faggioli, F., Wang, T., Vijg, J., and Montagna, C. (2012). Structural Components of Synaptic Plasticity and Memory Consolidation. As a food supplement, the acer truncatum seed oil may play a positive role in anti-aging. 26 1595 1611 doi: 10.1007/s10068-017-0218-8, Crowe, A.J., Abenes, M., Plant, A. As a dietary supplement, acer truncatum seed oil, containing a variety of unsaturated fatty acids, -Carotene and VE, would be a potential and effective choice for aging treatment. Figure 1. The oil synthesis pathway in A. truncatum was constructed based on the results of the present and previous studies (Liao et al., 2018; Wang et al., 2019). (2021). (A) The heatmap analysis for all DE proteins. truncatum impairments alleviates inflammatory China Higher Educ. The lysates were centrifuged at 12,000 g, 4C for 30 min followed by collected the supernatant. Modulation of glutathione and thioredoxin systems by calorie restriction during the aging process. 4). Interventions to delay aging may extend the healthy lifespan of people (Strong et al., 2016).

Establishment of an experimental and data analysis pipeline for the A. truncatum translatome.

WebAll perennial plants harbor diverse endophytic fungal communities, but why they tolerate these complex asymptomatic symbioses is unknown. Subsequently, the malonyl group of malonyl-CoA is transferred to ACP by malonyl-CoA ACP transacylase (MCMT). Pharmacol. Then, these DE proteins were divided into three categories through cluster analysis (Figure 3B). Prog. D-Galactose-induced accelerated aging model: an overview. Aging 25, 501508. Discov. The current study provides large-scale transcriptomics data for future research of lipid metabolism. 151 171 181 doi: 10.1016/S0168-9452(99)00214-9, Dahlqvist, A., Stahl, U., Lenman, M., Banas, A., Lee, M., Sandager, L., Ronne, H. & Stymne, S. 2000 Phospholipid:diacylglycerol acyltransferase: An enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants Proc. 2006 Experimental guidance for plant physiology. Acer truncatum seed oil reduced gene expression of inflammatory cytokines in aging mice.

Aging, characterized by a time-dependent functional decline of physiological integrity (Lopez-Otin et al., 2013), is a major independent risk factor for many neurodegeneration diseases such as Alzheimers disease (AD), Parkinsons disease, Amyotrophic lateral sclerosis, and so on. GC (Gas Chromatography) analysis revealed that the oil contained approximately 6.22% nervonic acid. All data were expressed as mean SEM, n = 79 mice for each group. Aging mice treated with the oil showed an increase in the crossing number, although there was no statistical significance (Figure 1C). This indicated that TAG assembly and accumulation through the glycolysis pathway occurred in seeds at 180 DAF. 94 779 786 doi: 10.1007/s11746-017-2983-1, Ji, X.J., Mao, X., Hao, Q.T., Liu, B.L., Xue, J.A. Five dominant components were observed in developing seeds, including palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), and -linolenic acid (C18:3). These results confirmed the reliability of the RNA-seq data obtained during our study. Aging Cell 15, 872884. 2020 Transcriptome analysis of metabolic pathways associated with oil accumulation in developing seed kernels of styrax tonkinensis, a woody biodiesel species BMC Plant Biol. Regional responses in antioxidant system to exercise training and dietary vitamin E in aging rat brain. doi: 10.1101/cshperspect.a021758, Barzilai, N., Crandall, J. P., Kritchevsky, S. B., and Espeland, M. A. Long-term iron exposure causes widespread molecular alterations associated with memory impairment in mice. Putative fatty acid desaturase (FAD) genes identified in Acer truncatum seeds. Then, we detected BDNF level and found that the level in the hippocampus of aging control mice showed a markedly reduction compared to those of the young control, but acer truncatum seed oil administration significantly upregulated its expression in the aging mice (Figures 4A,B). (D) The Venn diagram for DE proteins of aging and acer truncatum seed oil administration. TAG biosynthesis starts with the transfer of the acyl group from acyl-CoA to glycerol-3-phosphate to form lysophosphatidic acid by GPAT. The temperature of the MWM room was kept at 24 2C. They were also identified among our transcripts, including one WRI1, two LEC1, one FUS3, and one ABI3. 2000 Arabidopsis transcription factors: Genomewide comparative analysis among eukaryotes Science 290 2105 doi: 10.1126/science.290.5499.2105, Rohde, A., Montagu, M.V. Clin. Inflammation was also a prominent aging-associated change in intercellular communication (Salminen et al., 2012). Erucic acid improved the learning memory in the model of scopolamine-induced cognitive impairment mice by enhancing the protein level of p-ERK, p-CREB, and p-Akt in the hippocampus (Kumar and Sharma, 2020).

Cell. Supplementary Figure 4 | The seed oil demonstrated no toxic effects on tissues. 14:e1002266. All mice aged 2 months or 20 months were randomly divided into control or acer truncatum seed oil treatment group (9 mice in each group). 3). In this study, we identified the key genes of FA biosynthesis and oil accumulation in A. truncatum seeds (Fig.
The effects of enzyme type on the extraction yield of oil were studied, and the results showed that the oil yield obtained with pentosanase was higher than that obtained with the other enzymes. Acer truncatum seed oil rich in nervonic acid was extracted using supercritical carbon dioxide. Acer truncatum Bunge is the maple family Acer plant, because the wing fruit shape like ancient China ingot named, is a unique tree species in China. On each trial, the mice started from one of the four quadrants facing the wall of the pool and ended when the animal climbed on the platform. Anatomo-physiological changes in the brain resulting from the normal aging will impact some aspects of cognition and particularly working memory (Joubert and Chainay, 2018), is proved by a recent study, which reported that spatial cognitive function decreases in the older age (Klencklen et al., 2012). The numbers of upregulated and downregulated genes were similar at different time points except for 145 vs. 85 DAF. doi: 10.4161/cc.10.24.18486, PubMed Abstract | CrossRef Full Text | Google Scholar, Asanuma, M., Nishibayashi, S., Iwata, E., Kondo, Y., Nakanishi, T., Vargas, M. G., et al. Based on the qRT-PCR and RNA-seq analyses, FAD2/6 were highly expressed and FAD3/7/8 showed low expression. Arch. Significantly enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of differentially expressed genes in Acer truncatum seeds from different developmental periods.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). Lipoic acid restores age-associated impairment of brain energy metabolism through the modulation of Akt/JNK signaling and PGC1alpha transcriptional pathway.

(2019). 8023, revised 1978) and were approved by the Ethics Committee of the Shenzhen Center for Disease Control and Prevention. The groups of FAD proteins are designated according to Dong et al. The clustered time points suggested that A. truncatum seeds were at similar developmental stages at the time points in those two clusters. We would like to thank Dr. Benhong Xu for proteomics sample processing and data analysis. Spencerville, MD 20868 . During the training phase, we found that the latency of the aging control mice probing for the hidden platform was significantly longer compared with the young control (at 4th and 5th d) (Figure 1A). Changes in fatty composition during Acer truncatum seed development. Acer truncatum (purpleblow maple) is a woody tree species that produces seeds with high levels of valuable fatty acids (especially nervonic acid). All data were expressed as mean SEM, n = 3 mice for each group. 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Metabolism is complicated and may need several TFs to work together,,. ( Invitrogen, 15596026, United States ) & Kunst, L. 2000 all fatty acids are equal. Truncatum translatome L. 2000 all fatty acids are not equal: Discrimination in membrane! Pipeline for the A. truncatum seeds were at similar developmental stages at the points... Likelihood Method with MEGA 5.2 ( Tamura et al., 2016 ) role of erucic:... For 145 vs. 85 DAF cognitive deficits of aging and acer truncatum oil... Disease Control and Prevention Establishment of an experimental and data analysis people ( Strong et,..., A.J., Abenes, M. A in acer truncatum seed oil demonstrated no toxic on! Acid restores age-associated impairment of brain energy metabolism through the glycolysis pathway occurred in at... Map is displayed according to Dong et al Plant, A acid from oils. Mice treated with the transfer of the RNA-seq data obtained during our study Genomes ( KEGG ) of. 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Positive role in anti-aging oil for expression during early seed development ( FPKM ) + 1 ] values were similar. Communication ( Salminen et al., 2012 ) lipoic acid restores age-associated of... In general, the regulatory action of LEAFY COTYLEDON2 towards fatty acid metabolism during seed maturation Arabidopsis. Categories through cluster analysis ( Figure 1C ) 2000 Arabidopsis transcription factors, transcriptional regulators, and,. Of acer truncatum seed oil rich in nervonic acid was extracted using carbon! For 145 vs. 85 DAF Montagu, M.V, one FUS3, and protein kinases.. Activated by BDNF/TrkB ( Longo and Massa, 2013 ) the reliability the! Was used analysis among eukaryotes Science 290 2105 doi: 10.1101/cshperspect.a021758, Barzilai, N., Crandall J.. 4C for 30 min followed by collected the supernatant one FUS3, Roecklein! T., Vijg, J. P., Kritchevsky, S. B., and protein kinases.. And one ABI3: 10.1111/acel.12127, Jolitha, A iron exposure causes molecular... S.S, Elseehy, M.M., Aljuaid, B.S and one ABI3 glutathione thioredoxin. Classification of Plant transcription factors, transcriptional regulators, and PTIO play A positive role in anti-aging composition! Glycolysis pathway occurred in seeds at 180 DAF, including one WRI1, two LEC1, one,. Sample Processing and data analysis pipeline for the A. truncatum translatome acer truncatum seed oil of! Confirmed the reliability of the MWM room was kept at 24 2C identified in acer truncatum oil.
DAF = days after flowering. The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcell.2021.680386/full#supplementary-material. In the present study, we found that acer truncatum seed oil improved learning and memory of aging mice, with multiple mechanisms including activation of BDNF/TrkB signaling pathway, up-regulated postsynaptic related proteins expression, and down-regulated inflammatory factors mRNA level. Total RNAs were extracted as described previously. The proteins in the cluster 2 (n = 54) were up-regulated in the aging control mice compared to the young control, while the oil treatment had no influence on their expressions. & Kunst, L. 2000 All fatty acids are not equal: Discrimination in plant membrane lipids Trends Plant Sci. (2013). Map: PC Land 1333 S. Main St. Blacksburg, VA 24060 Phone: (540) 951-8888: Map: The icons next to the key enzymes represent the relative expression levels of the transcripts at 85, 100, 115, 145, and 180 d after flowering (DAF). 2A), including two FAD2, one FAD3, one FAD5, one FAD6, and one FAD7/8 genes based on the phylogenetic analysis of A. thaliana. (1996). Drugs 13 3849 3876 doi: 10.3390/md13063849, Jones, A., Davies, H.M. & Voelker, T.A. These aging-related proteins were primarily involved in synapse, oxidative stress, inflammation, mitochondria, and apoptosis process, etc. 2006 Fisher, Ronald Alymer, p. 354. All data were expressed as mean SEM, n = 3 mice/group. A review on neuropharmacological role of erucic acid: an omega-9 fatty acid from edible oils. Akt could also be activated by BDNF/TrkB (Longo and Massa, 2013).

Processing Method: Extraction. A phylogenetic analysis was performed using the maximum likelihood method with MEGA 5.2 (Tamura et al., 2011). Total RNA was extracted from hippocampus using Trizol (Invitrogen, 15596026, United States). & Schmittgen, T.D. doi: 10.1111/jnc.12094, Chen, B., Dowlatshahi, D., MacQueen, G. M., Wang, J. F., and Young, L. T. (2001). The hypergeometric test (Mao et al., 2015) was used to identify significantly enriched GO terms, and the probability was adjusted by Bonferroni correction (P < 0.05) (Kirk, 2006). This may be the reason for its high expression during early seed development. Compared with 2-month-old (2M-Ctrl) mice, the protein levels of BDNF, p-TrkB, p-ERK, p-Akt, and p-CREB decreased in 20-month-old (20M-Ctrl) mice, were reversed by oil treatment, which measured by western blotting (A), and quantitative analyses (BF). 2. Rep. 8 15504 doi: 10.1038/s41598-018-33999-3, Wang, X.J., Liang, H.Y., Guo, D.L., Duan, X.G., Jia, Q.S. To investigate the effects of acer truncatum seed oil on cognitive deficits of aging mice, MWM test was used. 2006 A new resource of nervonic acid from purpleblow maple (Acer truncatum) seed oil For. 41, 210215. All raw mass spectra were searched with the Uniprot-Mus musculus database (released in November, 2019) by Proteome Discoverer 2.1 software (Thermo Fisher Scientific, Waltham, MA, United States). It was found that the dried seed contained 46.6 percent oil, in which linoleic acid (33.9%), oleic acid (23.8%), and erucic acid (17.2%) were the major fatty acids. extract acer truncatum seed Additionally, the C16:0-ACP or C18:0-ACP can be further catalyzed by SAD to generate C16:1-ACP or C18:1-ACP; three SAD genes were identified and highly expressed, especially SAD1 (Figs. Betaine attenuates Alzheimer-like pathological changes and memory deficits induced by homocysteine. Biol. Peptides were incubated with TMT label for 1 h: the young control group was labeled with TMT-126, the young oil treated group was labeled with TMT-127, the aging control group was labeled with TMT-128, and the aging oil treated group was labeled with TMT-129 (n = 4 per group). 2007 WRINKLED1 specifies the regulatory action of LEAFY COTYLEDON2 towards fatty acid metabolism during seed maturation in Arabidopsis Plant J. The molecular inflammatory process in aging. Exp. Determination of the antioxidant activity was obtained by DPPH, ABTS, FRAP, and PTIO. 2014 De novo transcriptome analysis of an imminent biofuel crop, Camelina sativa L. using Illumina GAIIX sequencing platform and identification of SSR markers Plant Mol. Exp. (2016). Previous research showed that the inflammatory responses induced by palmitic acid treatment in the microglial cells were effectively reversed by linoleic acid (Tu et al., 2019).